The science behind a new, three-layer placental membrane


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The AmnioExcite placental membrane is available from Salvin Dental Specialties. (Photo: Salvin Dental Specialties)

Wed. 3. November 2021


The new AmnioExcite placental membrane, available from Salvin Dental Specialties, includes both amnion and chorion layers plus a trophoblast layer. According to the company, the trophoblast layer contains more than 50 percent of the biologically relevant proteins in the placental membrane,[1] and LifeNet Health’s patented and proprietary Matracell decellularization process serves to minimize immunogenic and inflammatory responses.[7,8]

The amnion membrane by itself is thin and contains only a small portion of the biological factors found in the full‐thickness placental membrane, Salvin said. The chorionic membrane contains many of the same growth factors as the amnion membrane. However, the trophoblast layer holds a majority of the proteins and growth factors in the full‐thickness placental membrane.[1]

AmnioExcite is approximately four times thicker than an amnion or chorion membrane for a more substantial protective barrier.[2] AmnioExcite’s unique thickness may help prevent bacterial ingress and thus inflammation associated with infection, the company said.

AmnioExcite does not fold in upon itself when rehydrated, making it much easier to handle and less likely to migrate away from the surgical site during the healing process.[3]

Unlike other amnion/chorion membrane allografts, the layers in AmnioExcite are never separated during the entire process because separation can adversely impact retention of the native growth factors.[4] In most placental‐based allografts, the trophoblast layer is removed. However, by removing this layer, a rich source of beneficial endogenous biological factors and the thick structural matrix is also removed.[1] Therefore, AmnioExcite retains more endogenous growth factors than similar placenta‐derived allografts by being processed as an intact, whole placental membrane, according to Salvin.

A few tissue processors do retain the trophoblast layer without removing viable and non‐viable cells, which may provoke an immune response because of the presence of antigens on the cell surface.[5] The ideal solution is to leave the thick, nutrient-rich extracellular matrix of the trophoblast layer intact but remove the donor cells to minimize an immune or inflammatory response.

LifeNet Health has solved this problem by processing AmnioExcite with its Matracell decellularization process.[6,7] Matracell is a patented and validated process that renders allograft tissue acellular without compromising the biomechanical or desired biochemical properties of the allograft bio‐implant for its intended surgical application.[8]

Both Salvin Dental and LifeNet Health have an exemplary safety record. Since 1995, LifeNet Health has processed more than 7 million bio-implants with its Allowash technology, without a single case of disease transmission. AmnioExcite has a Sterility Assurance Level (SAL) of 10‐6, which is the recommended SAL for medical devices or allografts that are implanted or come in contact with breeched skin.

To learn more about AmnioExcite or to place an order, call Salvin Dental Specialties at (800) 535-6566.


  1. LifeNet‐Health. TR‐004‐2020 Characterization of the Amnion, Chorion, and Trophoblast Layers of Decellularized and Freeze‐Dried Placental Membrane. 2020.
  2. Verbruggen SW, Oyen ML, Phillips AT, Nowlan NC. Function and failure of the fetal membrane: Modelling the mechanics of the chorion and amnion. PLoS One. 2017;12(3): e0171588.
  3. LifeNet‐Health. ES‐20‐057 Data on File. 2020.
  4. QinXC, Silvia; Aschenbach, Lindsey; Chen, Jingsong, inventor; LifeNetHealth, assignee. Decellularized placental membrane and methods of preparing and use thereof. USA2018. 6,743,574 Full-Text Process for devitalizing soft-tissue engineered medical implants, and devitalized soft-tissue medical implants produced.
  5. Organogenesis.‐wound‐care‐nushield‐patient‐brochure.pdf 2019.
  6. Moore MA, Samsell B, Wallis G, Triplett S, Chen S, Jones AL, et al. Decellularization of human dermis using non‐denaturing anionic detergent and endonuclease: a review. Cell Tissue Bank. 2015;16(2):249‐259.
  7. LifeNet‐Health, inventorUS2018/0361026A1 pending. USA2018.
  8. Moore MA, Samsell B, Wallis G, Triplett S, Chen S, Jones AL, et al. Decellularization of human dermis using non‐denaturing anionic detergent and endonuclease: a review. Cell Tissue Bank. 2015;16(2):249‐259.

(Source: Salvin Dental Specialties)

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